Journal: Stem Cells and Development
Article Title: α5β1 Integrin Promotes Anchoring and Integration of Transplanted Stem Cells to the Trabecular Meshwork in the Eye for Regeneration
doi: 10.1089/scd.2019.0254
Figure Lengend Snippet: Expression of fibronectin in murine TM. (A) Immunostaining of fibronectin (red) in the TM of normal mice. The positive signal for endothelial cells of Schlemm's canal and vessels displaying green (CD31 staining). (B) Fibronectin staining (red) in the TM of laser-treated mice at 2 weeks. DAPI stains nuclei blue. Scale bars 50 μm. (C) Quantification of mean fluorescent intensity of fibronectin expression in normal and laser-treated mouse TM in three to five samples. *P < 0.05. Unpaired t-test. CO, cornea; SC, Schlemm's canal. Color images are available online.
Article Snippet: Fibronectin (FN) coating group: 0.5 mL of fibronectin and collagen (FNC)-coating solution (Athena ES, Baltimore, MD) containing 10 μg/mL FN was coated at room temperature for 3 h on 8-chamber slides.
Techniques: Expressing, Immunostaining, Staining